Control of the Staining Procedure after Paper Electrophoresis
نویسندگان
چکیده
منابع مشابه
Rapid lipid-staining procedure for paper electrophoretograms.
Paper electrophoresisiswidely used in clinicalmedicine as a screening test for hyperlipidemia. It is a simple, reproducible procedure, but requires 36 h to complete. Aliquota of sera are electrophoresed 16-18 h, paper strips are dried for 30 mm, followedby overnightstaining(1, 2). Recently it was reported that stainingtime could be decreased to 2 h by using Oil Red 0 stain in acetone-water solv...
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UR PRESENT KNOWLEDGE of human serum lipoproteins and their significance in atherosclerosis may be attributed to the development of suitable methods (1, 4, 6, 8, 9, 13, 15) and to extensive studies of human subjects (2, 5, 10, 11, 16, 18) by such methods. The ultracentrifugal, the chemical, or the free electrophoretic methods are technically difficult for routine determinations of serum lipoprot...
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15 صفحه اولPaper electrophoresis as a quantitative method: the staining of serum lipoproteins.
Electrophoresis of serum on filter paper followed by staining 3 of the lipoprotein fractions with lipid dyes is technically the simplest method for the separation and estimation of serum lipoproteins. A number of procedures for the staining of separated lipoproteins on paper with various lipid dyes have appeared (1-8), but the only comprehensive consideration of the quantitative aspects of dye ...
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ژورنال
عنوان ژورنال: Nature
سال: 1956
ISSN: 0028-0836,1476-4687
DOI: 10.1038/177586a0